The purpose of this study was to seek ways to improve the quality of life of workers by separating workers into the low fatigue group and the severe fatigue group depending on the level of fatigue level, and analyzing and understanding the difference between quality of life and job stress according to the fatigue level. Methods: For the ‘Development of Mibyeong management system which was a consumer-directed health care service’, the data of job stress, fatigue and quality of life was collected from 115 workers who visited the oriental hospitals located in J city and C city from Jul, 2012 to Dec, 2012. Results: The severe fatigue group showed significantly higher job stress than the low fatigue group in physical environment(p=.004), job demand(p<.001), interpersonal conflict(p=.012), lack of reward(p=.062). The severe fatigue group showed lower degrees both in physical component summary(PCS)(p<.001) and mental component summary(MCS)(p<.001) measured by SF-12 and the quality of life(p<.001) measured by EQ5D than the lower fatigue group. A relatively strong negative correlation was found in the item of mental component summary(MCS) measured by SF-12. Conclusion: Considering our results that high fatigue level show negative impact on quality of life and job stress, we suggest to provide a variety of health promotion strategies to improve the quality of life through the prevention and relief of fatigue for workers.